Date: 6th – 11th April 2014
Location: Rothamsted Research, Harpenden, UK
This popular RMS course covers all aspects of cryo-techniques for electron microscopy and is aimed at biological sciences, but the methods can be transferred to other fields. The intensive five day course teaches sample preparation and cryo-microscopy techniques for both scanning and transmission electron microscopy. Participants from both academia and industry who wish to learn new skills or update their knowledge are encouraged to attend.
Techniques covered will include the Tokuyasu method, rapid freezing of samples, freeze substitution, cryo-sectioning, thin film TEM and cryo-transfer and freeze fracture cryo-SEM. Attendees will have the opportunity to use state of the art equipment and instrumentation from a range of manufacturers.
Tutorials, demonstrations and hands-on practical sessions will be led by technical experts from Gatan, Leica, Diatome and Jeol. Keynote speakers will supplement the techniques taught.
Plenary speaker – Professor Helen Saibil (Birkbeck College)
Cryo fixation – Kent MacDonald (University California Berkeley)
Cryo sectioning – Helmut Gnaegi (Diatome)
CryoSEM – Kim Findlay (John Innes Centre)
CryoTEM – Dave Bhella (Glasgow University)