NO news is good news - Detection, Measurement and Quantification of Nitric Oxide: Tools for Nitric Oxide Research

Proceedings from the 23rd March 2007 meeting in Hertfordshire, UK

“Nitric oxide (NO), generated by the enzyme nitric oxide synthase (NOS) plays a key role in a diverse range of physiological and pathophysiological conditions within the cardiovascular, immune, reproductive and nervous systems. Since NO is a small, diffusible, highly reactive free radical with a short half-life, and is present in low concentrations, real-time detection of NO is extremely difficult. This meeting will examine methods used to detect and visualise NO-producing cells. Furthermore, direct and indirect techniques used to measure NO will be examined, including among others, the measurement of NOS enzymatic activity, electron paramagnetic resonance (EPR) and NO-sensitive electrodes. Finally, tools that facilitate NO research, such as NOS inhibitors will be examined”. Meeting’s Chair: Dr Nicholas Warrick – University of Oxford

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Table of Contents

Preface

Contents

Introduction

NITRIC OXIDE
NITRIC OXIDE SYNTHASE
ACTIONS OF NITRIC OXIDE
MEETING OUTLINE
REFERENCES

ORAL PRESENTATIONS

Quantification of Blood and Tissue NO Metabolites by Gas Phase Chemiluminescence: a Guide to NO Man’s Land

Introduction
Vascular Nitric Oxide Metabolism
Methods for Metabolite Measurement
Principle of Chemiluminescence
Methods of Sample Preparation
Controversy
Practicalities
Concluding Remarks
References

Radiochemical HPLC measurement of nitric oxide synthesis (NOS) activity in vascular tissue

EPR-based measurement of reactive nitrogen intermediates in human inflammatory diseases

Nitric oxide sensors and their biomedical applications

Understanding NO synthase inhibition; potency, selectivity and implications for their pharmacology

References

Endogenously occurring inhibitors of nitric oxide synthase

Abstract
Methylated arginines as NOS inhibitors
Measuring ADMA
Conditions in which ADMA is elevated
Arginine methylation
Regulation of ADMA
Could ADMA be causative in cardiovascular pathologies?

Evaluating endothelial function in humans: From bench to bedside

Nitric oxide synthase inhibition and renal injury

References

Quantification of enzyme independent stores of nitric oxide in human skin

References

POSTER PRESENTATIONS

NO quantification in abnormal and manipulated red blood cells

References

Application of NO detection using Fe(DETC) spin trap and ESR spectroscopy in pharmacological research

Halogenated volatile anestherics (HVA)
Flavonoids. Baicalein and luteolin
TO-85 and TO-133
Mildronate
References

Hypoxia-induced regulation of nitric oxide synthase in cardiac endothelial cells and myocytes

Methods
Results
Conclusions

Nitrite regulation of energy expenditure in man

Nitric oxide inhibits cellular lipid and protein synthesis without energy depletion: a process of chronic diseases due to nitrosothiols

NOx concentrations in human brain extracellular fluid and their relationship with energy metabolism: a pilot microdialysis study in acute brain injury