| Friday, October 13, 2006 9:00 am - 4:30 pm | ||
HSP peptide activated NK cells in the immunotherapy of cancer - DrGabriele Multhoff University Hospital Regensburg, Germany Leukemic blasts frequently present Hsp70 on their cell surface. In contrast, normal bone marrow and PBL were found to be Hsp70 membrane-negative thus indicating that membrane-bound Hsp70 serves as a tumor-selective recognition structure. We demonstrated that an incubation of NK cells with low dose IL-2 plus Hsp70 peptide TKD, acting as a danger signal, initiates the cytolytic activity of NK cells against autologous and allogeneic blasts. Extra-cellular heat shock proteins - functions and uses - HSP70 chaperone machine remodels protein aggregates at the initial step of HSP70-HSP100 dependent disaggregation - Pawel Stocki The proper conformation of proteins is challenged by stress conditions. Exposure of cell to extreme heat shock conditions results in a massive protein aggregation. Process of solubilization and reactivation of aggregated proteins in cell is mediated by two cooperating chaperone systems: Hsp70 (DnaK, DnaJ and GrpE) and Hsp100 (ClpB). Based on green fluorescent protein (GFP), as an experimental substrate model and chaperonin mutant GroEL D87K, called ‘trap’, as a tool I was able to examine and characterize the individual steps of disaggregation process, dependent on both Hsp70 and Hsp100. Our results suggest that Hsp70 chaperone system monomerizes aggregated GFP at the initial step of disaggregation reaction. These monomers aggregate secondarily in the absence of Hsp100. HSP70-hom gene polymorphism and the risk of post-transplant complications in the recipients of allogeneic haematopoietic stem cell transplantation - Dr Katarzyna Bogunia-Kubik Institute of Immunology and Experimental Therapy, A number of genetic polymorphisms have been shown to be associated with the outcome after allogeneic haematopoietic stem cell transplants (HSCT). The talk will summerise the results of the studies on genetic markers within the HSP70 encoding genes in recipients and donors of allogeneic stem cells and their impact on the transplant outcome. In more detail, our findings on the relationship between the HSP70-hom gene polymorphism and the risk of post-transplant complications, susceptibility for generation of severe toxic lesions and acute graft-versus-host disease (aGvHD), will be presented and discussed.
Hsp70 analysis - role in haematopoietic stem cell transplantation - Prof. Anne Dickinson University of A human skin explant assay has been used in our laboratory since 1988 to both predict GvHD and study the immunobiology of the disease. This talk will summarise the use of the assay to study HsP70 in lesions of GvHD using the assay and discuss the implications of the results for predicting the disease. The duo "Hsp70- O-GlcNAc" in cell protection - a brake to pathologies development - Céline Guinez Recently we have shown that Hsp70 and Hsc70 were endowed of a lectin property toward GlcNAc residues. Intriguingly, this lectin activity is dependent upon stress conditions. A detailed study of Hsp70/Hsc70 lectin properties for various glucose concentrations has shown that Hsp70 presented a high affinity for GlcNAc until 5 mM in glucose, whereas Hsc70 presented a higher affinity at this concentration. Interestingly these two proteins are themselves O-GlcNAc modified, but the glycosylation did not influence the GlcNAc binding properties. Our results tend to demonstrate a link between HSP70 lectin properties, O-GlcNAc glycosylation and proteasomal degradation resistance. The role of hsps in the innate immune recognition of bacterial pathogens - Dr Kathy Triantafilou University of The focus of the Infection and Immunity Group at the |
Investigating cellular stress responses October 13 2006