Friday, September 29, 2006   9:30 am - 4:30 pm
 

 

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Talks include

Capturing more of the proteome

Professor David O’Connor 

Proteomic approaches are typically used to answer four key questions: (1) which proteins are present?  (2) what does this protein interact with?  (3) which proteins change under conditions of interest? (4) where are these proteins localized within cells or tissues?  Currently, efforts to address these questions are hampered by proteome complexity and the vast dynamic range of protein expression. Accordingly, we have been developing new approaches for capturing a larger proportion of the expressed proteome.  The talk will focus on the use of these methods to increase the proportion of the serum proteome that can be routinely sampled.

 

Biomarker discovery using proteuslims™ - the proteomics lab information and scientific data management system

Mr. Kevin Jones


LI-COR Odyssey Imaging System: The new standard in protein quantification and Western blot analysis

Speaker to be confirmed


LI-COR Imaging systems are based on detection in the Infra-red region of the spectrum, which confers many significant advantages for the researcher.  This talk will outline the LI-COR technology and further brief you on the advantages of Infra-red fluorescence detection.  In addition to quantitative 2 colour Western analysis the Odyssey is capable of many other applications where it's Infra-red capability plays a key advantage along with time and overall cost savings.  The talk will also cover some of these additional applications such as In Cell Westerns or Cell based assays, Tissue Section Imaging, Small Animal Imaging, Protein arrays and much much more.

 

Fishing for Alzheimer's Disease Biomarker Candidates with 2-D Gel-Based Expression Proteomics - Dr Timothy Cross

Alzheimer's disease is an age-related disorder affecting more than 30% of people over age years of age. In this study we have applied a 2-D gel-based expression proteomics workflow to compare the brain profiles between 3 month-old control and APP23 transgenic mice which exhibit Alzheimer's disease like neuropathologies at 9 months, but not at 3 months, of age. We shall show data that demonstrates differential protein expression patterns between these two groups and how the application of novel protein sample preparation tools and techniques can enhance proteome coverage and enrich for low-abundance proteins.

Advances in the imaging and analysis of Cy dye-labelled 2D gels

Dr Paru Oatey
The Dyversity imager can capture 2D gel images from fluorescent and visible stained gels. It utilises a cooled, mega-pixel camera that excels at resolving the high density and large dynamic range of proteins found on a typical 2D gel. Its unique darkroom has fully computer controlled lighting and camera settings accessed through the GeneSnap acquisition software.

Faster imaging times can be achieved compared to conventional laser scanning and scanning camera

systems.

 

Dymension is revolutionary software that can analyse a typical 2D gel image rapidly. It features novel algorithms for background subtraction, noise filtering, precise warping, spot detection and spot matching. With Dymension, the entire analysis process from background correction to spot matching results takes minutes, making this the fastest 2D gel analysis package currently available.

 

 "Revealing Hidden Treasure", Applying Intact Protein Partitioning and Fractionation to Biomarker Discovery

Dr John Thorn,

 

Proteomics: Challenges and Technologies