Friday Nov 24th 2006, London
Talks include
Towards
improving innate immune robustness in the chicken - Dr P Kaiser- Head
of the Avian Genomics group at the Institute for Animal Health, Compton,
Oxon
The availability of the chicken genome sequence provides the opportunity
to resolve outstanding questions concerning which molecular components of the
immune system are shared between mammals and birds, and which represent their
unique evolutionary solutions. One benefit of this approach should be to
identify genes whose products drive innate immune responses in birds, and
subsequently single nucleotide polymorphisms (SNPs) in these genes between
different lines of chickens that differ in their resistance to pathogens. SNPs
that correlate with disease resistance are potential markers for use in
marker-assisted selection programmes, with the aim of breeding chickens with
improved innate immune robustness.
Comparative
Immunology – mapping disease resistance genes - Professor Michael Stear
- University of Glasgow
We now have the technology to map disease resistance
genes although identifying the causative mutations is proving more difficult.
This talk will discuss how to identify the genetic basis of disease resistance
using both quantitative and molecular approaches.
Trypanosomiasis in mice and cattle; a model for the response to
acute infection
Dr Harry Noyes -
Trypanosomiasis caused
by Trypanosoma congolense is a major constraint on cattle production in Africa.
We have mapped 5 QTL in mice and 10 in cattle that are associated with response
to infection. The challenge is now to identify the genes underlying these QTL.
We are using gene expression microarray, tiling array and SNP data to identify
associations with infection and visualising this data in the context of known
gene networks to identify those which are involved in the response to infection
as well as the networks that respond differently to infection in susceptible and
resistant animals.
Cytokine gene discovery in fish by in silico analysis -
Dr Chris Secombes
Many homologues of known mammalian cytokines are
known to exist in fish. However, cytokines with no obvious homologues are also
apparent, whilst some mammalian genes, such as those belonging to the Th2
cytokine cluster, have not been found. Whilst a complex cytokine network is
clearly present in fish, particular duplications may have led to fish specific
or tetrapod/higher vertebrate specific genes arising in different vertebrate
classes. This talk will illustrate this by analysis of cytokine genes of the
classical proinflammatory cascade, and the interferon gamma
locus.
Making sense of cellular interactions in mucosal
tissues - Dr Michael
Bailey –
In the post-genomic era, we are now in a position to make direct, functional comparisons between immunological mechanisms in different species, since direct, molecular equivalences can be demonstrated. So, studies of the behaviour of CD4+ T-cells in the intestinal mucosa of the pig are relevant to humans, since the evolutionary relationships between the molecules can be determined. The level of immunogical organisation of the pig intestinal mucosa is more obvious than that of humans or rodents, and the impact of commensal flora after birth is more pronounced. Using multiple, fluorochrome-tagged monoclonal antibodies allows studies of the interactions between cells in this tissue.
Exploiting innate immunity to improve the health and performance
of pigs. - Dr Stephen Bishop, Div. Genetics and Genomics Roslin
Institute (Edinburgh)
Understanding the genetic control of innate immunity in
domestic animals is of fundamental scientific interest and strategically
important. We have demonstrated that several easily measured innate immune
traits, e.g. white blood cells counts and acute phase protein concentrations,
are both heritable and genetically related to performance in growing pigs. Such
measurements may provide the means of improving the health and performance of
animals in situations where disease problems are difficult to define. This talk
will outline this strategy and describe our approaches to utilising SNP markers
to attempt to locate the genes underlying variability in innate immune
measurements.