Tuesday, May 09, 2006 - 9:00 am - 5:00 pm
 
Birkbeck College 
Basement Lecture Theatre 
43 Gordon Square 
London,    WC1H 0PD  
United Kingdom 


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Talks include:

 

PCR applications in medical microbiology

Dr Craig Winstanley, Department of Medical Microbiology and Genitourinary Medicine, University of Liverpool 

An overview of the main PCR-based techniques of relevance in medical microbiology.  Brief description of PCR-based subtractive hybridisation and its application to designing diagnostic PCR tests for specific cystic fibrosis pathogens.


Real-Time Reverse Transcription PCR and molecular staging in Colorectal Cancer

Professor Stephen A. Bustin, Institute of Cell and Molecular Science, Barts and the London, Queen Mary's School of Medicine and Dentistry, University of London

The detection of lymph node (LN) metastasis constitutes the most important prognostic factor in colorectal cancer and as the primary indicator of systemic disease spread, LN status determines the choice of postoperative adjuvant chemotherapy. However, its limitations are emphasised by the considerable prognostic heterogeneity of patients within a given tumour stage: not all patients with LN-negative cancers are cured and not all patients with LN-positive tumours die from their disease. This has resulted in a search for more accurate staging protocols and has seen the introduction of the concept of �molecular staging�, the incorporation of molecular parameters into clinical tumour staging. One such parameter is the qRT-PCR assay�s potential for generating quantitative results that are not only more informative than qualitative data, but contribute to assay standardisation and quality management.


Int22h-related inversions causing haemophilia A: a new more discriminant PCR test for their detection.

Dr Richard Bagnall- Department of Medical and Molecular Genetics, King's College School of Medicine, Guy's Hospital , London

Multiplex Real-time PCR assays for human infectious disease detection

Dr Vicki Chalker - Respiratory and Systemic Infection Laboratory, Health Protection Agency Centre for Infections, London

Diagnosis of foot-and-mouth disease by RT-PCR at the IAH Pirbright: a review

Dr Scott Reid - Institute for Animal Health, Pirbright Laboratory

 

A brief review of the development and evaluation of improved RT-PCR methodologies for routine diagnosis of foot-and-mouth disease (FMD) at the World Reference Laboratory (WRL) for FMD in Pirbright is presented. The talk will provide an introduction to the disease and the causative virus followed by discussion of the RT-PCR methods evaluated at the WRL over the last 7-8 years. These include conventional, immuno-capture, PCR-ELISA and real-time formats. Present RT-PCR research topics will be discussed along with assay validation, future objectives and the impact made by the 2001 epidemic on the development of RT-PCR methodologies for diagnosis of FMD.


Comparison of three multiplex cytokine analysis systems: Luminex, SearchLight and Fast Quant

Dr Gendie Lash, School of Surgical and Reproductive Sciences, University of Newcastle-upon-Tyne


Health Protection Agency Centre for Infections Campylobacter and Helicobacter Reference Unit London

Dr Stephanie Chisholm - Implementation of conventional and real-time PCR for routine diagnosis of Helicobacter pylori infection and antibiotic resistance.


Additional confirmed speakers include

  • Dr Jim Huggett - Centre for Infectious Diseases & International Health, University College London
  • Dr Scott Reid  - Institute of Animal Health, Pirbright, Surrey
  • Dr Iturriza-Gomara M, Enteric  - Virus Unit, Virus Reference Division, Centre for Infections, Health Protection Agency, London

 

Improving Clinical & Diagnostic PCR