Discussion Workshop: Stem Cells - 6th May 2011


The Penridge Suite, 470 Bowes Road, London, N11 1NL, United Kingdom: 6th May 2011

This event is discussion workshop. We have invited 6 experts to discuss their work in an informal lecture setting, discussion and demonstration groups, one2one sessions and panel discussions

Meeting Chair: Dr Glyn Stacey, UK Stem Cell Bank, Division of Cell Biology and Imaging.

 

This event has CPD accreditation

On registration please submit your questions to the panel that will be asked by the chair on the day of the event

 

9:00 – 9:30 Registration

 

9:30 – 9:45 Introduction by the Chair: Dr Glyn Stacey, UK Stem Cell Bank, Division of Cell Biology and Imaging

Talks by Invited Experts:

 

9:45 – 10:00 Cardiomyocytes from Human Pluripotent Stem Cells

Dr Chris Denning

Associate Professor & Reader in Stem Cell Biology

University of Nottingham, UK

The ability to maintain human pluripotent stem cells (hPSCs) for extended periods of time in culture and yet induce their differentiation to a wide range of cell types offers new opportunities in biomedicine. We will discuss our progress in producing human induced pluripotent stem cells from patients with genetic heart disease and our efforts to model disease using in vitro derived hPSC-cardiomyocytes, including in drug evaluation. We will also consider issues of scale up and defined culture / differentiation.

 

10:00 – 10:15 Differentiation of HESC/iPS

Dr Nadire Ali

NHLI, Imperial College London, UK

 

10:15 – 10:30 Characterisation of Human Pluripotent Stem Cells

Dr Paul Gokhale

Research Associate

Centre for Stem Cell Biology Sheffield, UK

Characterisation of pluripotent stem cells is critical if reliable cell lines are to be created for drug screening and therapeutic uses. This talk will describe the characterisation of human embryonic stem cells that has been carried out by the International Stem Cells Initiative at the level of gene expression, imprinting, methylation, karyotype and copy number variation. Attention will be drawn to key properties that mark bone fide human ES cells

 

10:30 – 10:45 Principles of Cryopreservation

Dr Charlie Hunt

Operations Manger

Uk Stem Cell Bank, National Institute for Biological Standards and Control

An essential pre-requisite to the commercial and clinical application of stem cells are suitable and effective protocols for cryopreservation and long-term storage. Whilst effective methods for cryopreservation have been developed for haematopoietic and mesenchymal stem cells, embryonic and iPS cells have proved more refractory. Effective cryopreservation requires an understanding of the basic physical principles that underly the freeze/thaw process and the cells response to the imposition of such a process. Basic principles and their application to stem cells will be discussed.

 

10:45 – 11:00 Defined Media

Mr Paul Bello

Stem Cell Sciences

 

11:00 – 11:15 Cell Adhesion and stem cell growth and differentiation

Expert TBC

 

11:15 – 11:20 Participant Photo

 

11:20 – 11:45 Mid-morning Break

 

11:45 – 12:45 Question and Answer Session

 

12:45 Working Lunch (in Exhibition Area)

 

13:00 – 16:30 Discussion Groups and One to One Sessions

 

  • Round table discussion groups will be throughout the afternoon
  • Delegates will rotate at 15 minute intervals so that they may participate in all the discussion tables
  • All delegates will also be allocated an slot to visit the exhibition stands
  • One to one sessions can also be held after lunch (in parallel to the discussion groups)
  • Where appropriate delegates will be able to bring their samples to the discussions

 

Table 1: Sourcing stem cell lines: Points to consider

Hosted by Glyn Stacey

 

Table 2: Culture and Differentiation of Human Pluripotent Stem Cells

Hosted by Chris Denning, PhD in Cancer Gene Therapy at Beatson Institute for Cancer Research, University of Glasgow, 1997; Postdoctoral Research Fellow - gene targeting in mouse ES cells, Institute for Stem Cell Research, University of Edinburgh, 1997-1998; Postdoctoral Research Fellow - gene targeting in somatic cells; first targeted gene disruption in animals other than mouse, Roslin Institute 1998-2001; Principal Investigator, University of Nottingham, 2001-2003; Medical Research Council Fellow in Stem Cell Biology, University of Nottingham, 2003-2006; Lecturer in Stem Cell Biology, University of Nottingham, 2006-2008; Associate Professor & Reader in Stem Cell Biology, University of Nottingham 2008-

Table 3: Differentiation of HESC/iPS

Hosted by Nadire Ali

 

Table 4: Characterisation of Human Pluripotent Stem Cells

Hosted by Dr Paul Gokhale, PhD , Tumour Cell Biology, 1999. Postdoctoral researcher with Prof. PW Andrews, University of Sheffield (1999-2005). International Stem Cell Initiative (2005-present). ISCI-1(2005-2008): Characterisation of Human embryonic stem cells and ISCI-2(2008-2011): Identification of common genetic changes in Human embryonic stem cells.

Table 5: Problems in Cryopreservation of Stem Cells

Hosted by Dr Charlie Hunt, whose background has been in the fields of cryobiology and tissue banking. In 1978, he joined the MRC Medical Cryobiology Group at the University of Cambridge where his research involved the cryopreservation of cornea, pancreatic islets, and arteries. .In 1992, he and colleagues founded the East Anglia Tissue Bank; where he was responsible for the heart valve and autologous stem cell banking programs and developing skin and amniotic membrane banking. In 2003, he took charge of the newly founded UK Stem Cell Bank at the National Institute for Biological Standards and Control, where he is currently the Operations Manager.

Table 6: Defined Media

Hosted by Mr Paul Bello

Table 7: Cell Adhesion and Stem Cell Growth and Differentiation

 

16:30 – 17:00 Expert Panel’s Summing Up

 

17:00 Chairman’s Summing Up

 

Key words:

automation, human pluripotent stem cells, disease modelling, cardiomyocytes, genetic modification, hES cells, iPS cells, freezing, vitrification, cryopreservation

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This meeting was organised by Euroscicon (www.euroscicon.com), a team of dedicated professionals working for the continuous improvement of technical knowledge transfer to all scientists. Euroscicon believe that they can make a positive difference to the quality of science by providing cutting edge information on new technological advancements to the scientific community. This is provided via our exceptional services to individual scientists, research institutions and industry.

 

Meeting Web Site: http://www.regonline.co.uk/WORKSHOPStem2011

 

 

 

 

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