Vivantis Flex -C Cloning kit is a highly efficient, rapid and easy to use PCR cloning kit. The Flex-C Enzyme allows direct cloning of any PCR fragments to any linearized expression vector at any site in a single 20-minute reaction.
The application protocol is simple. The PCR fragments can be generated by PCR Polymerases with primers that are designed to have at least 10 bases of homology at their linear ends. No additional treatment of the PCR fragment is required. The linearized vector can be generated by PCR or restriction enzymes. Flex-C Enzyme joins PCR fragments and linearized vectors accurately and efficiently by recognizing the 10bp overlap at their ends.
This method allows cloning multiple fragments into a single vector in a single reaction without sub-cloning. Flex-C Cloning Kit is highly efficient with 95% insert rate. It also includes Vivantis Taq DNA Polymerase and reaction buffers as well as dNTP for subsequent PCR screening of clones.
Vivantis is a research-based biotechnology company who, for many years have specialized in the production of Restriction Enzymes, DNA extraction kits, DNA amplification reagents and other related products for molecular biology research. The Company has strong markets in Asia and the USA and has recently opened a European centre to help expand their activities and support a growing customer base.
Says Vivantis “now anyone can clone a PCR fragment in a single 20 minute reaction…!”