Proteomics/Mass Spectrometry, Department of Biochemistry, University of Cambridge, Central Cambridge.
We are seeking to employ an enthusiastic postdoctoral scientist to join the Cambridge Centre for Proteomics at the University of Cambridge, led by Professor Kathryn Lilley. The successful candidate will work on a project funded by a joint Wellcome Trust Investigator award to Professor Anne Willis (MRC Toxicology Unit, University of Leicester) and Professor Lilley. The project is entitled “Transcription, Trafficking, Translation: dissecting the spatiotemporal mechanisms underlying localised protein synthesis”.
It is now widely acknowledged that mRNA is spatially organised such that its translation frequently occurs in the vicinity where the corresponding protein product adopts its function. This phenomenon has been studied extensively in specialised cells, however, recent research has suggested that spatial restriction of mRNA storage and translation is much more widespread.
The aims of this project are to define where translation of mRNA takes place in the cell, how this is regulated by protein binding partners and sequence motifs and how this changes upon cellular perturbation.
We are building a team of RNA biologists, mass spectrometrists, and informaticians to help tackle these challenging aims.
This post involves the creation of RNA correlation profiling methods, including sub cellular fractionation, cross linking of RNA to binding proteins and next generation sequencing.
The ideal applicant will have (or in the final stages of completing) a PhD in Biological Sciences with expertise in RNA biology, translation, cell biology, cell fractionation and next generation sequencing methods. Some knowledge of mass spectrometry would also be advantageous, along with an interest in informatics and the handling of large datasets.
The following recent publication has direct relevance to this project:
1. A draft map of the mouse pluripotent stem cell spatial proteome. Christoforou et al Nat Commun. 20167:8992. doi: 10.1038/ncomms9992
Fixed-term: The funds for this post are available for 5 years in the first instance.
To apply online for this vacancy and to view further information about the role, please visit:
http://www.jobs.cam.ac.uk/job/10411. This will take you to the role on the University’s Job Opportunities pages. There you will need to click on the ‘Apply online’ button and register an account with the University’s Web Recruitment System (if you have not already) and log in before completing the online application form.
Informal enquiries can be made to Professor Lilley – firstname.lastname@example.org
Please quote reference PH09166 on your application and in any correspondence about this vacancy.
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